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C4585
Sigma
Monoclonal Anti-β-Tubulin−Cy3 antibody produced in mouse
clone TUB 2.1, purified immunoglobulin, buffered aqueous solution
| Synonym: | Monoclonal Anti-β-Tubulin |
Description
| Immunogen | tubulin from rat brain. |
| Legal Information | Cy is distributed under license from Amersham Biosciences Limited. |
| Specificity | The antibody recognizes all five isoforms of β-tubulin (β1-β5).1 It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Cy3 is a trademark of GE Healthcare |
Properties
| antibody form | purified immunoglobulin |
| clone | TUB 2.1 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 55 kDa |
| species reactivity | chicken, human, frog, wheat, plant, rabbit, bovine, mouse, hamster, moth, rat, sea urchin |
| storage condition | protect from light |
| application(s) | direct immunofluorescence: 1:100 using cultured chicken fibroblasts or BHK cells |
| isotype | IgG1 |
| Gene Information | human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383) |
| rat ... Tubb2(29212), Tubb2c(296554) | |
| mouse ... Tubb1(104068), Tubb2a(22151), Tubb2c(227613) | |
| shipped in | wet ice |
| storage temp. | 2-8°C |
Safety
| WGK Germany | 3 |
References
| Cited Reference | 1. Wolff, A., et al. Biol. Cell 63, 319-326, (1988) Abstract |
| reference | Karabay, A., et al., Regulation of Microtubule Severing by Katanin Subunits during Neuronal Development J. Neurosci. 25, 5573-5583, (2005) Abstract |
| Hao, H., et al., Limitations of allotopic expression of mitochondrial genes in mammalian cells. Genetics 165, 707-720, (2003) Abstract | |
| Karabay, A., et al., The Microtubule-severing Proteins Spastin and Katanin Participate Differently in the Formation of Axonal Branches Mol. Biol. Cell 19, 1485-1498, (2008) Abstract | |
| Gozes, I., et al., Monoclonal antibodies that recognize discrete forms of tubulin. Proc. Natl. Acad. Sci. U. S. A. 79, 2579, (1982) | |
| Matthes, T., et al., Antitubulin antibodies. II. Natural autoantibodies and induced antibodies recognize different epitopes on the tubulin molecule. J. Immunol. 141, 3135, (1988) | |
| Schomai, A., and Shaul, Y. Hepatology 37, 764-770, (2003) Abstract | |
| Soroka, C.J., et al. J. Biol. Chem. 274, 26416-26424, (1999) | |
| Gingras, D., et al. J. Biol. Chem. 271, 12807-12813, (1996) | |
| Binarova, P., et al. Plant Cell 12, 433-442, (2000) Abstract |
